RBS

Part:BBa_K5280413:Design

Designed by: Bowen Shi   Group: iGEM24_HKUST-GZ   (2024-09-09)


A RNA thermosensor that turns on protein translation at high temperatures.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Increasing stem length optimizes the RNA thermosensor for higher temperatures, while decreasing stem length has the opposite effect. The stem sequence was also changed to improve thermosensor sensitivity.


Source

Improved based on iGEM18_Jilin_China

References